Analysis of 62 Chalcolithic individuals from sites across Romania, Hungary, Italy, and the Czech Republic provides a valuable window into population dynamics during the Copper Age. The genomic signal is dominated by Neolithic farmer ancestry — the legacy of Anatolian-derived agriculture — combined with local Western hunter-gatherer (WHG) admixture. This blend is consistent across many samples, reflecting long-term demographic continuity in central and southern Europe.
Y-chromosome haplogroups observed include G (6), I (5), and J (2), with single occurrences of CT and C; these lineages are frequently associated with farmer and local European ancestries in the Neolithic and Chalcolithic. Mitochondrial diversity is notable for haplogroups J (11), H (7), N (5), U (5), and T (4), highlighting maternal continuity with earlier farming populations and some regionally variable maternal inputs. Importantly, while most individuals carry farmer-derived genomes, a subset shows increased affinity to steppe-related ancestry in the later part of the dated range (approaching c. 2500 BCE), suggesting limited admixture events rather than wholesale population replacement.
With 62 samples the dataset is moderately robust, but geographic clustering (e.g., dense sampling in certain burial sites) can bias regional reconstructions. Where sample counts are low for particular sites, conclusions about local dynamics remain provisional. Overall, genetics and archaeology together indicate a mosaic of continuity, local admixture, and episodic external contacts during the Chalcolithic.