Five ancient individuals from Arroyo Seco II provide a rare genetic window into early Holocene South America. The Y-chromosome profile is dominated by haplogroup Q (4 of 5 males), a lineage widely associated with Native American paternal ancestry and inferred to descend from the Beringian-derived founding population(s). On the maternal side, mitochondrial haplogroups include C1b (2), D1 (1), A2 (1) and D1g (1). These mtDNA clades are among the canonical founding lineages observed across the Americas.
Together, the paternal and maternal signals are consistent with ancestry that traces to the initial peopling of the continents, followed by regional diversification in South America. Genetic affinities to other early South American individuals point toward broad relatedness rather than extreme isolation. Yet with only five samples, statistical power is low: patterns of substructure, sex-biased migration, and long-term continuity cannot be robustly resolved.
Archaeogenetic analyses can test hypotheses raised by material culture—such as whether mobility corridors inferred from artifacts correlate with gene flow—but for Arroyo Seco II conclusions must remain tentative. Future sampling, particularly larger numbers and comparisons across neighboring sites, will be essential to move from suggestive patterns to confident reconstructions of demographic history.