Six ancient mitochondrial genomes recovered from Mayahak Cab Pek and Saki Tzul show a diversity of maternal lineages: A, A2q, C1c, C5b, and an R-lineage. These haplogroups are broadly consistent with Native American maternal diversity: A and C subclades are widely observed across ancient and modern populations in the Americas. The presence of an R-class mtDNA is notable but should be interpreted cautiously: macro-haplogroup R includes many sublineages and the designation here may reflect a basal or poorly resolved branch rather than direct links to Old World R clades.
No robust Y-chromosome signal is reported for this dataset or available Y-DNA was insufficient for population-level inference. With only six samples (<10), population genetic inferences are necessarily tentative. Preliminary analyses suggest genetic continuity with other early Mesoamerican and Isthmo-Colombian groups in broad strokes, but fine-scale structure (local subgroups, kinship patterns, sex-biased mobility) cannot be confidently reconstructed.
Future sampling—both more individuals and targeted nuclear DNA—would clarify ancestry proportions, relatedness among buried individuals, and connections to later Maya populations. For now, the genomes anchor these sites within the long-standing Indigenous genetic landscape of Central America while underscoring the need for larger, carefully contextualized datasets.