Genome data from four individuals recovered at Cañada de la Virgen provides a slender but valuable genetic window into this medieval Guanajuato community. Maternal lineages are represented by mtDNA haplogroups A (two individuals), D1m (one), and B2 (one). These mtDNA lineages are well-established in Indigenous American populations: haplogroup A is widespread across North and Central America, B2 is common in Mesoamerica and the Andes, while D1m represents a deeper sub-branch whose interpretation benefits from larger comparative datasets. The predominance of A among these four individuals is consistent with regional maternal diversity, but the sample is too small to infer population-level frequencies with confidence.
Paternal markers show one individual assigned to haplogroup Q — a lineage widely associated with Indigenous peoples of the Americas — and one assigned at a broader level to haplogroup F. The F assignment should be treated cautiously: in some ancient datasets a call to F can reflect low-resolution Y-chromosome data or upstream placement before finer downstream assignment. Contamination, coverage limits, and analytical thresholds can affect Y-chromosome interpretation, especially with few male samples. Overall, the genetic signal aligns with an Indigenous highland population carrying pan-American maternal haplogroups and at least one Q paternal lineage; however, with only four genomes (<10), conclusions about population structure, sex-biased migration, or continuity with later groups remain preliminary and tentative. Future sampling and high-coverage sequencing will be required to test kinship within burials and demographic scenarios suggested by the archaeology.