Sixteen individuals from southern Bulgaria (1100–500 BCE) provide a preliminary genetic window into Early Iron Age populations of the region. Among these, 13 yielded assignable mitochondrial haplogroups: H (6), U (3), K (2), H+ (1) and J1c (1). The predominance of haplogroup H among maternal lineages is consistent with broad Eurasian European continuity from the Neolithic onward; K and J1c are often associated with Neolithic farmer expansions, while U lineages can reflect older hunter‑gatherer ancestry persisting in the gene pool.
Y‑chromosome data for this dataset are not reported or are limited, so paternal lineage patterns remain unresolved here. Nonetheless, broader ancient DNA studies across the Balkans show that Early Iron Age populations frequently carry mixed ancestries: Anatolian‑Neolithic farmer heritage, Western hunter‑gatherer legacy, and varying proportions of steppe‑related input associated with Bronze Age migrations. Archaeological evidence of cultural change in Kapitan Andreevo, Stambolovo, Diamandovo and Svilengrad is therefore plausibly echoed by a genetic tapestry of continuity plus incoming elements.
Interpretation should be cautious: geographic sampling is focused in southern Bulgaria and the sample size, while larger than many initial studies, remains limited for capturing fine‑scale structure. Further Y‑DNA results and broader regional sampling are needed to clarify male‑line dynamics, migration routes and social practices affecting gene flow such as marriage patterns and mobility.