Genomic data from 92 individuals spanning Croatia, Hungary, Czech Republic, Italy and France afford a broad, though regionally uneven, view of Chalcolithic population dynamics. Y‑DNA counts show a predominance of haplogroup G (19) and substantial representation of I (14), both commonly associated with Neolithic farmers and local Mesolithic continuity in Europe. The smaller numbers of R lineages (4) suggest limited presence of steppe‑associated paternal ancestry in this dataset, while rarer C (3) and CT (1) lineages point to occasional diverse paternal inputs. Because haplogroup counts do not directly equate to ancestry proportions, caution is needed when inferring demographic processes from them alone.
Mitochondrial diversity—dominated by H (13), U (11), K (10), T (10), and a notable T2b subset (7)—reflects a mixture of farmer‑associated maternal lineages (H, K, T) and lineages often linked to hunter‑gatherer ancestry (U). This balance supports an interpretation of substantial Neolithic farmer continuity admixed with local hunter‑gatherer gene pools. Across the region, genetic signatures align with archaeological complexity: sites linked to Lasinja, Baden or Vucedol horizons show varying degrees of local continuity and incoming influence.
With 92 samples the picture is robust relative to many ancient datasets, but geographic sampling remains uneven. Therefore, while broad trends (farmer majority with hunter‑gatherer admixture and limited steppe signal) are supported, local nuance and later transitions (e.g., Bell Beaker, Early Bronze Age) require denser sampling and careful temporal resolution.