Three ancient DNA samples from La Hoya dated between 400 and 173 BCE provide a very small window into the genetic makeup of this Iron Age community. Of these three individuals, one carries a Y-chromosome in haplogroup I — a paternal lineage that has deep presence across parts of Europe. Mitochondrial haplogroups observed include H1, J, and U (one individual each), all of which are well-attested in prehistoric and historic European populations.
These haplogroups do not by themselves prove population origins or movements; they are markers that, when combined with genome-wide data, can illuminate ancestry components. Broad ancient DNA research across Iberia shows mixtures of local Mesolithic/Neolithic-descended lineages with later influxes of steppe-associated ancestry during the 3rd and 2nd millennia BCE, and continued regional differentiation into the Iron Age. Archaeological context at La Hoya suggests local continuity, and the mitochondrial diversity (H1, J, U) is compatible with long-standing maternal lineages in the region.
Crucially, with only three samples (<10), any genetic conclusions remain preliminary. Limited evidence suggests local continuity with ties to wider Iberian and European genetic backgrounds, but larger sample sizes and genome-wide comparisons are needed to clarify migration, kinship networks, and sex-biased processes in Iron Age Basque Country.