Ancient DNA from 46 individuals associated with the Latte context at Garapan provides one of the clearer genetic windows into late precontact Mariana Island populations. The maternal lineages are dominated by haplogroup E subclades: E2a (27 individuals), E (13), E2 (5), and a single B lineage. This distribution suggests strong matrilineal continuity with broader Austronesian-derived populations of Island Southeast Asia and parts of Near Oceania, where mtDNA E variants are known to occur. The presence of a B lineage is not unexpected given regional mtDNA diversity and possible interactions across ocean networks.
Crucially, common Y-chromosome haplogroups were not reported for these samples, so paternal ancestry patterns remain unresolved; without Y-DNA or genome-wide autosomal summaries, inferences about sex-biased migration, admixture, or later colonial-era male-mediated gene flow are limited. With 46 samples, these mtDNA patterns are statistically informative compared with very small series, but interpretation still requires caution: sampling is concentrated at Garapan, temporal spans cover several centuries, and post-contact demographic disruptions could have reshaped genetic landscapes. Archaeogenetic evidence aligns with archaeological signals of Austronesian cultural roots while leaving open questions about the timing and directionality of specific gene flow events.