Three genomes from Pikutkowo provide a slender but valuable genetic window into TRB peoples in Poland. Key uniparental markers observed here are: one Y‑DNA lineage assigned to haplogroup C, and mtDNA haplogroups U5a, N, and H3t (one sample each). Because only three individuals were sequenced, these observations should be treated as preliminary patterns rather than population‑level frequencies.
The presence of mtDNA U5a is noteworthy: U5 subclades are commonly associated with Mesolithic and post‑Mesolithic Western hunter‑gatherers in Europe, suggesting retention or integration of forager maternal ancestry within TRB groups. Haplogroup N and an H subclade (H3t) may reflect maternal inputs linked to Neolithic farmer lineages or later local diversification; the specific affinities of H3t remain unclear with this small dataset.
Y‑DNA haplogroup C is rare in Neolithic Europe and its occurrence at Pikutkowo could indicate: (a) a vestigial forager‑derived paternal line, (b) long‑distance mobility or exchange, or (c) stochastic survival of a low‑frequency lineage. Autosomal ancestry in contemporaneous TRB individuals elsewhere shows a mixture of Anatolian farmer and Western hunter‑gatherer ancestry with little Steppe input before the third millennium BCE; Pikutkowo is consistent with this broader picture, but robust autosomal conclusions require larger sample sizes.
In summary, genetic data from Pikutkowo hint at a mosaic ancestry in which farmer and forager lineages coexisted. Additional samples and wider geographic sampling are needed to resolve population structure and migratory dynamics.