BAM/CRAM to T2T Alignment

Yes. We accept BAM or CRAM files previously aligned to any common reference genome build (hg19/GRCh37 or hg38/GRCh38), indexed or unindexed. The original reads are extracted and then re-aligned from scratch to the T2T-CHM13 reference using the optimal algorithm for your read type.

Processing typically takes 24-48 hours depending on file size and current queue. Whole genome sequencing files at 30x coverage usually complete within 48 hours. We'll notify you via email when your results are ready for download.

You will receive a coordinate-sorted BAM file aligned to T2T-CHM13, along with its index file (.bai). We also provide alignment statistics including mapping rate, coverage distribution, insert size metrics (for paired-end data), and quality score distributions. All files are delivered via secure download link.

This service is intended for research and ancestry purposes only. It is not designed, validated, or certified for clinical diagnostics, medical decision-making, or health-related interpretations. For clinical applications, please consult with appropriate certified laboratories and healthcare providers.